83 



of the 3'east-cells through bacteria or moulds). After three days' 

 incubation in eanesngar, inocnhited in flask B with 0,5 perc. glycose, 

 0,5 perc. peptone, 0,05 perc. MgSO^ and a trace of KNO,. In flask /I, 

 filled with the same fluid, also beer-yeast from the same source, 

 which, however, has already twice been transplanted on a phosphate- 

 free medium, and does not contain any more volutin. After 18 hours 

 both mercury columns fallen 4| c.c. (to the mercui-y level in the 

 reservoir). ^) In a subsequent similar experiment both fallen 2 c.c. 

 after one hour's fermentation. 



Reduction tests after Bb^nedict. 



July 1915. A 20 c.c. culture fluid with glycose, 0,05 perc. MgSO, 

 and a trace of KCl. B similar fluid -}- O/^ perc. KH^PO^. 



Before the fermentation determination of reduction = 0,5 glycose. 



A inoculated with volutin-free, B with volutin-containing culture 

 of Torula monosa. After 48 hours' fermentation 



^ = 0,15 perc. glycose. 



B =. 0,15 perc. glycose. 



March 1917. A 20 c. c. of culture-fluid with glycose, 0,5 perc. 

 asparagin and a trace of KNO3. ^ similar fluid -|- 0,1 perc. KH^PO^. 

 Before fermentation determination of reduction = 2,25 perc. glycose. 



A inoculated with volutin-free culture, B with volutin-containing 

 culture of Torula monosa. After 48 hours in A still 2.06 perc. glycose. 



in B also 2,06 perc. glycose. 



March 1917. A 15 c.c. of culture-fluid with glycose, 0,2 perc. 

 asparagin, 0,05 perc. MgSO^ and a ti-ace of KNO3. B similar fluid 

 -f- 0,1 perc. KHjPO^. A inoculated with volutin-free, B with volutin- 

 containing culture of Lactose-yeast. Before the fermentation determ- 

 ination of reduction = 2,04 perc. glycose. After 48 hours in 

 A still 1.53 perc. glycose. 

 B 1.44 perc. glycose. ^) 



It cannot be doubted therefore, that with Torula monosa, Saccha- 

 romyces cerevisae and Lactose-yeast fermentation takes places in- 

 dependently of volutin, the same holding perhaps for other 

 zymase-containing hypho- and blastomycetes. It is not surprising that 

 in some experiments the fermentation in the phosphate-containing, 

 control-cultures is somewhat stronger in the same space of time. As 

 I stated before, the growth of yeast is more intense on a phosphate- 

 containing medium, so that the number of the cells, participating 

 in the fermentation- process must be more considerable. We also 



1) See preceding note p. 82. 



2) I am endebled to Mr. J. W. Bi:st for the last two determinations. 



6* 



