90 

 Some of the observations referring to this are united in table II, 



TABLE II. Determination of the quantity of alkali fixed by 

 glycine and alanine. 



D 



H 



80 cm3 of a solution containing + 5 "/o glucose 



A sohition of glucose in water of fixed ooncentration was under 

 comparable circumstances submitted to the action of different quan- 

 tities of alkali. The number of cm', normal NaOH-solution added 

 varied between and 10 cm', per 100 cm^ solution. 



The most important decrease and the darkest yellow colour occurred 

 there, where most alkali had been added (experiments G and 1). 

 After about two hours the polarisation of G and I had diminished 

 fVom + 10,9 to + 8,3 and -\- 8,5. By the experiments H and J 

 was proved once more the protective influence of glycine and alanine; 

 the polarisation was respectively + 10,0 and + 9.6. 



The polarisation of H lay between that of C and D. From this 

 it is apparent that 500 milligram glycine has fixed 10 — 3^ = 6,5 cm\ 

 1,06 N.NaOH = almost 7 cm\ N.NaOH. The intensity of colour 

 was in accordance with this. In an analogous way it was demonstrated 

 that 500 milligrams of alanine had fixed about 5 >; 1,06 = 5,3 cm'. 

 N.NaOH. 



Glycine and alanine regarded as monobasic acid, 500 milligrams 



