186 



in boiling water. With hydrochloric acid the colour became jellow, 

 with sodium hydroxide, ammonia or soda violet and otherwise. So 

 the colour acted as indicator. 



Furthermore I observed that the infection with Fusarium occurred 

 too with solutions of amygdalin, on which Aspergillwi niqer had 

 developed first (temperature 34°). 



Twelve days after inoculation with Aspercjfilhis niger 1 delivered 

 the solutions in question containing amygdalin from the mould 

 layer; the clear solutions were kept at ordinary temperature in 

 tumblers covered with watch-glasses. Not all amygdalin was used, 

 a part had remained. 



Miss Prof. Dr. Joh. Westerdi.ik was kind enough to determine 

 the isolated Fusarium. The found species was Fusarium discolor 

 var. triseptatum ^). 



This organism was found first by Sherbakoff on rotting potatoes. 



It was possible that in my case the descent would be the same, 

 because a few^ years consecutively I had experimented on potatoes 

 in the same laboratory. 



"With the isolated species of mould I made almost the same 

 researches as some time ago with Aspergillus niger. ") 

 TABLE I. (Fusarium). 



Glucose as exclusive organic 

 food. 



Amygdalin as exclusive organic 

 food. 



Composition of the culture liquid : 50 cm^ of tapwater, in which dissolved 



0.15 /o NH4NO3, 0.150/0 KH2PO4, 0.1% magnesiumsulfate (crystallised). 



Ordinary temperature. 



A. 20/0 glucose: 1000 milligr. 



B. 2 o/o amygdalin : 1000 milligr. 



Assimilated 

 glucose 

 (milligr.) 



Obtained 



dry weight of 



mould 



(mgr.). 



Number of 



days after 



inoculation. 



Assimilated 



amygdalin 



(milligr.). 



Obtained 



dry weight of 



mould 



(milligr.). 



1000 



1000 

 1000 



322 



297 

 300 



274 

 347 



235 

 299 



1) G. D. Sherbakoff, Fusaria of potatoes. Memoir N". 6. Cornell University 

 Agricultural Experiment Station, May 1915. p. 239. 



2) Amygdalin as nutriment for Aspergillus niger., These Proceedings Vol. XIX, 

 p. 922 (1917). 



