466 



Temperature I. Pat. F. R. 



Febr. March. 



24 25 26 27 28 1 2 3 4 5 6 1 8 9 10 11 12 13 14 



On March 2 I drew by means of a svringe ± 20 c.c. of blood 

 from a brachial vein (of course with the necessary aseptic j^recau- 

 tions). Immediately I transferred Vz to 1 c.c. of the blood to tubes 

 filled with 10 c.c. respectively of tapwater, 0.25 "/„ NaCl, 0.57o 

 NaCl, 0.75 7o NaCl., peptonewater, ascites-glycerin-bouillon, (1 c.c. 

 ascites fluid, 9 c.c. of bouillon, 0.4 c.c. of glycerin) and 2 7o ^™roo- 

 nium oxalate, two tubes of each sort. The fluids were heated to 

 body-temperature and in going from the laboratory to the hospital 

 and vice versa I toolt preventive measures to insure security from 

 cooling down. I made use of water and several amounts of common 

 salt, because, if Nicolle, Conor and Conseil are right in asserting 

 that the virus is contained chiefly in the leucocytes, I could thereby 

 liberate the organisms by destroying the leucocytes. According to 

 GoLDBERGER aud Anderson, however, who made similar experiments 

 to those of the researchers just mentioned, and who pointed to their 

 faulty conclusions, it may be more readily assumed that the virus 

 occurs isolated in the plasma. However this may be, I thought fit 

 to take note of both investigations. 1 grew at 37° C organisms both 

 in aerobic and anaerobic cultures. To obtain the latter 1 put some 

 pyrogallol and potassium-hydrate upon the cotton plug and shut up 

 the tubes with rubber stoppers. Of course I also made the necessary 

 control-experiments. 



After intervals of 24 hours preparations were made of all aerobic 

 cultures; the anaerobic cultures were examined only macroscopically, 

 to see if there was any growth. After 3 X 24 hours preparations 



