467 



were made also of the anaerobic cultures. The aerobic cultures 

 yielded the following results: 



Blood in water: a few diplobacilli. Gram positive. 



Blood in 0.25 "/„ NaCl : a few diplobacilli, Gram positive. 



Blood in 0.5 V„ NaCh a few diplobacilli. Gram positive. 



Blood in 0.75 7o NaCl : no micro-organisms. 



Blood in peptone- water: no micro-organisms. 



Blood in ascitesglycerin-bouillon : a few diplobacilli. Gram positive. 

 ' Blood in ammonium-oxalale: a few^ diplobacilli. Gram positive. 



From the tubes, in which diplobacilli had been found cultures 

 were made upon sloped agar. 24 hours later everything was examined 

 again. The agar-tubes had all remained sterile. In the other tubes 

 the following was observed : 



Blood in water: a few diplobacilli, to all appearance not many 

 more than the first time. Gram positive. 



Blood in 0.25 7o NaCl : many diplobacilli. Gram positive. 



Blood in 0.5 " „ NaCl: many diplobacilli. Gram positive. 



Blood in 0.75 Vo NaCl : no micro-organisms. 



Blood in peptone-water: no micro-organisms. 



Blood in ascitesglycerin-bouillon : rather many diplobacilli. Gram 

 might be called positive as well as negative. 



Blood in 2 7o ammonium-oxalate: some diplobacilli, not many 

 more than the first time. Gram positive. 



From the tubes in which diplobacilli were found again cultures 

 were made upon sloped agar. 



After 24 hours it appeared that besides the remains of red blood- 

 corpuscles, also diplobacilli, very much like those found previously, 

 were present in the liquid expressed from the agar of the agar-tubes, 

 inoculated with the blood -f 0.25 NaCl and the blood -f 0,5 NaCl, 

 likewise in the liquid of the agar-tubes that had been inoculated 

 the day before. All the other agar-tubes had i-emained sterile. The 

 preparations of the liquid-tubes were similar to those of the 

 second day. 



The anaerobic tubes appeared yet to be sterile. 



The question now arose if what had been found in the fluid 

 squeezed from the agar, meant growth or whether it had been 

 evolved by inoculation. I collected the fiuid from the several tubes 

 and inoculated it into sonrje sloped-agar tubes, made a stabculture 

 and added broth to the rest to the ratio of 1 expression of water 

 to 10 of broth. After 24 hours, preparations were made of the 

 broth and many diplobacilli were found. 



When watching the agar-tubes, especially those that had been 



