469 



temperature they do not grow at all. At first a culture was made 

 every day; after some experiments the interval could be lengiheued, 

 at most to about 3 weeks, this being the longest interval after which 

 growth seems to be possible. 



Upon gelatin at 22° the growth is exceedingly tardy. No lique- 

 faction of gelatin takes place. 



Upon Löffler's serum: rather poor growth; especially the above- 

 mentioned granules are made out here. 



Upon Endo the growth is also poor. The colonies are white and 

 change to light red after a few days. 



In bouillon a sediment is formed. The broth does not get 

 evenly turbid, only in tlie lower layers. 



Indol is not produced. Milk does not coagulate. Saccharose-, 

 lactose-, mannite-, glycose-, maltose-, raffinose-bouillon -. no acid- 

 formation, no fermentation. 



In caviae, inoculated with a suspension of this broth, a rise of 

 temperature was observed after 4 or 5 days, that persisted for a 

 shorter or a longer space of time as is shown in List 11. 



Celsius 

 39° 



Temperature II. Cavia. 



Subcutaneous injection of a suspension of the bacilli. 



They then sat quietly in a corner of the hutch and appeared to 

 be ill. To a second injection a short time after the temperature had 

 become normal, there was no response and the temperature remained 

 constant. To all appearance they had become immune as is shown 

 in List 111. 



In order to make sure that this was not brought about by a less 

 pathogenic condition of the cultures, also healthy caviae were 

 inoculated at the same time. With them a reaction really took place. 



We managed to cultivate the same bacilli with the same properties 

 from the blood drawn from the heart of one of the caviae by 

 means of a puncture and that on a day on which the temperature 



