Celsius 

 39° 



38° 



37° 



36° 



470 

 Temperature III. Cavia. 





• Subcutaneous injection of a suspension of the bacilli. 

 *" Inoculated for the second time. 



was very high. This experiment was conducted in the same way 

 as described above. 



Lastly I have also been able to study the agglutination. On the 

 9'^ of March, i.e. 7 days 'after the blood had been drawn for the 

 culture, I again drew some blood from the patient. The serum 

 agglutinated the bacilli in a J : 100 dilution; the serum of two 

 sound subjects and a sick one (with a high temperature) only in a 

 1 : 25 dilution. In a second patient, who had also suffered from 

 typhus exanthematicus I performed an intravenous puncture 11 days 

 after the crisis (See List IV). I did not succeed in cultivating bacilli 

 from the blood. The serum agglutinated the bacilli described above 

 in a 1 : 100 dilution. The history of the case is briefly as follows: 



17 Febr. pat. (M. J. F. V. 21 years) was down with a bad 

 head-ache, gripes, fever, pains in the arms and legs, chills. For 

 about 3 weeks previously he had been languid. Status Pi^aesens: 

 pulse: quite full, not dicrotic; Coniiinctiva slightly red. Diffuse 

 bronchitis in both lungs ; patellar reflexes very high, spleen did not 

 seem to be enlarged,- as appeared from percussion, not palpable. 

 Scattered roseolae on chest, back, arms, legs, belly, also some in 

 the face. Hemorrhage from the mucous membrane of the mouth ; 

 here and there spontaneous bleeding. The urine presents nothing 

 peculiar. Number of leucocytes 8000. Widal negative. Diagnosis: 

 spotted typhus. 



It appeared, then, that the serum of both patients, one of whom 

 was recuperating, agglutinated the bacilli in a 1:100 dilution; the 

 serum of a patient suffering from another disease with high fever 

 in a 1 : 25 dilution ; the serum of sound subjects also 1 : 25. 



In connection with the communications of da Rocha Lima and 

 TöPFER I have examined some clothes-lice, taken from the body- 

 linen of the first patient (R). I prepared as well as possible the 

 digestive and intestinal tract from the body and ground it on an 



