ied 
(69) 
the grouping of the lower organisms seems unable to level the 
differences between counting- and culture-method. 
To explain this there are but two possibilities, Lstthe greater 
number of lower organisms occurring in human faeces cannot develop 
under the given conditions (alkalin gelatin at 22° C.), or 2nd the 
excessive majority of the microscopically visible bacteria have died, 
or at least, are so much attenuated in their vital functions as no more 
to be fitted for multiplication. 
In order to test the former explanation on the experiment, the 
bacteria of the faeces were allowed to multiply under various ex- 
ternal circumstances, whose influence on the development of other 
bacteria-species is commonly known; among these are: a. the nature 
of the nutrients, 5. the reaction of the medium; c. the absence or 
presence of free oxygen, and d. the temperature of growth. 
EA Bak? BIE. 
a ' N ESO = = = 
F5 E u = 8 ERS _ | Number of bact. found by culture and Sa 
5 S én ese te 8 5 4 estimated for 1 mgr. of faeces. os 
& oa fone i Sg 2 aie et ws ' + A En 
Da “er 3 Py O tS 3 A) © 3 En 
on on dh DE a ie OIS O8 A, se 
© © oo Se, Se Ge ASO je) Onnen : oe 
er; 5 EEn | SSEO So + EE ie) Oa 
ie SS HSS Shy Ea wm wes bo Pd 
6 Heo [EE SRS Ee | AR PE dt Sa 
=| + = = = SPOE A a NO 
2 | 88 |SE| Es [A88 Be [Se | & $2 
SP a Wo" GE ie n= 5 Ay 
| | aya 
1 | 10.830 | 15.70 | 77.602000 6.396000 5.299000 2.735000 12 
| aes 
2 | 10.480 | 15.49 | 45.299000 | 692300) 552900) 754800 605800 65 
3 | 41.327 | 23.88) 31.7(9000) 101000, 89500, _ 115900 24100) 314 
A 12 270 | 16 59 | 86.972000 7410 2450 7410 9780, 11737 
5 11.495 | 20.13 | 60.363000 | 432000) 394000) _ 595000 307000 139 
6 11 185 | 24.83 | 47.662000 12700, = 11700) 13200 12700} 3753 
| 12.110 | 24.72 | 20.162000} 379. 300) 300700, 35800 45600 53 
8 10 780 | 30.60 74.959000 356 0 2848 1068) 210559 
9 10.735 18. 66 | 39. 9610C0 6400 5000 3600 5400; = 6244 
From Table II we see that colonies, developing on alkalin gelatin 
and acid malt gelatin at 22° C, on !/, pCt. glycose-gelatin under 
anaérobic relations, and on agar-agar at 37° C., are different in 
1) Arpx. Kinin, Kin Apparat zur bequemen Herstellung von anaéroben Platten- 
culturen. Centralbltt. fiir Bact. u. Paras. le Abth, Bd. XXIV, 1898, s. 967, 
