(215) 
hydrolysis into glucose and urea and the latter again into carbon 
dioxide and ammonia. 
The lowering of the freezing point gave an average molecular weight 
of 216, the calculated figure for Cy Hy. 0O;.N.CO.NH, being 222. 
Of the physical properties the following are of importance. 
The specific gravity was determined at 25° by the method of floa- 
ting im a dense liquid using mixtures of amylalcohol and ethylene 
bromide. That of glucose-ureide was found to be 1.48 and that of 
glucoseanhydride 1.544. Assuming that of urea to be 1.31 being the 
average of 1.3 (BOEDEKER) and 1.323 (SCHRÖDER) we find the mole- 
cular size of: 
urea glucose glucose-ureïde 
60 180 222 
—— = 46 — — 117 ——— 15 
ane 1.54 oo 
A slight expansion, therefore, takes place during the formation of 
the ureide from its components with liberation of water. 
CO(NHe)o -+ Cs His Os = Cs Hie O; EN 00. NH, + H,O 
(46) (117) (150) (18) 
The heat of combustion as determined with BEeRTHELOT’s calori- 
metric bomb was found to be 3742 calories per gram or 8307 K. 
per gram-molecule. The heat of combustion of glucose amounts to 
6736 K. per gram-molecule, that of urea 1525 K., so that the reaction 
is accompanied by a heat absorption of 49 K. 
Co Hs Os — CO(NH)s — Ce Hie O; e N . CO ° NH, + H, 0 zen 49 C. 
Chemical properties. The reducing power towards FrrLiNG’s solu- 
tion is maintained, but the time of boiling being equal it was less 
pronounced than in the case of glucose. It is in fact a secondary 
phenomenon which is preceded by the dissociation of the glucose- 
ureide by aqueous soda at the boiling temperature. Then if the solu- 
tion of glucose-ureide is reduced alkaline with ‘aqueous soda and 
mixed in the cold with copper sulphate, the copper hydroxide is 
dissolved with a dark blue colour. Unlike glucose this solution is 
“not reduced at the temperature of the room but only after a few 
moments boiling. A slightly acid solution of copper acetate (BARFOED’s 
reagent) is not reduced by glucose-ureide, a proof that the aldehyde 
group is no longer present. 
In order to form osazone, glucose-ureide requires a more prolonged 
heating with an acetic acid solution of phenylhydrazine than glucose. 
On adding sodium acetate, the formation of osazone is still more 
retarded. 
