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By oxidation with sodiumhypobromide the ureïde is very slowly 
attached at the temperature of the room and it takes hours before 
the evolution of nitrogen has quite ceased. 
On heating to 50°, it ceases in a few minutes. The total quantity 
of nitrogen evolved amounts in both cases to nearly the theoretical 
quantity. Both atoms of nitrogen are therefore liberated. 
By the action of nitrous acid the urea-group is completely elimi- 
nated from the glucose-ureide molecule (partly as ‘nitrogen and partly 
as ammonia) but at the ordinary, or a more elevated temperature, 
the liberated glucose is also simultaneously attached. The decompo- 
sition proceeds, however, normally and almost quantitatively by passing 
gaseous N.,Os; into the aqueous solution of the ureïde cooled to 0°. 
This is, therefore, a convenient way for recovering sugar from its 
combination with urea. 
In the first communication it has already been suggested that the 
reaction of glucose and urea might be a reaction of limits. This has 
now been confirmed by the hydrolytic dissociation of glucose-ureide 
by dilute acid into glucose and urea. It appeared that this reaction 
does not proceed to the end but that an equilibrium limit is reached 
which at the same temperature and the same concentration of the 
acid equals the equilibrium limit at which the condensation of urea 
and sugar remains stationary. 
The action of alkali at an increased temperature was finally found 
to also cause a hydrolytic dissociation. At 25° this is, however, not 
the case or only in a very small degree, but still the solution at 
that temperature undergoes a rapid and strong change in rotatory 
power. This change seemed to be dependent on the temperature but 
not on the concentration or nature of the alkali. The amount of 
alkali after a few days action at 25° was found to be unchanged. 
It was, therefore, suspected that a partial shifting of atoms of the 
glucose-ureïde had taken place. 
This was confirmed as fully 75 percent of glucose-ureide was reco- 
vered unchanged from the solution and also a syrupy substance which 
appeared to have nearly the same percentage of nitrogen (11.5) as 
glueose-ureïde but a much higher specifie rotation ([a]p — about + 39°). 
It is, therefore, highly probable that it is a mixture of ureides of 
other aldoses formed from glucose by the shifting of atoms. 
By acetylation of glucose-ureide with acetic anhydride and a 
trace of zine chloride a fine crystalline acetyl derivate was obtained 
(m.p. 200°) which on analysis was found to contain five acetyl 
groups. As very probably one of the acetyl groups in placed near 
the urea-group (the substance does not react with alkaline solution 
