(414) 
in unlimited quantity and without admixtures, should perhaps give 
more satisfactory results. 
My request to Prof. PawLow for some further information about 
the method of operating, was answered with the greatest kindness 
by a very detailed and accurate account by Dr. WALTHER about 
everything that must be taken into consideration when applying 
the gastral fistula and oesophageal fistula, according to PAWLOw’s 
method. Prof. Narata then kindly applied for me in a dog of 
25 KG., first a gastral fistula and when the wound was completely 
healed and the silver cannula was fixed in it, also an oesophageal 
fistula. Besides to the gentlemen already mentioned, I am also 
greatly obliged to Messrs. DE BRUIN, SCHIMMEL and THOMASSEN, 
lecturers at the State Veterinary School at Utrecht, who when the 
good healing of the wound in the stomach was in some danger, 
repeatedly gave me excellent assistance. 
Throughout the whole of the year 1901 the dog, who was always 
in an excellent state of health, was regularly used, generally three 
times a week, for the producing of gastric juice. By a pseudo- 
feeding with meat there was obtained in the first hour an average 
of 200 ce, in the second hour still an average of 100 cc. of gastric 
juice. Each time the fluid obtained in a quarter of an hour, was 
filtered in order to free it from little flakes of mucus. The filtrate 
was then perfectly colourless and clear. Only occasionally when 
the amount of pepsin was great and the temperature of the room 
was low, it showed some opalescence, which however, when heated 
to the temperature of the body, disappeared at once without leaving 
a trace, to reappear again when cooled. The amount of acid was 
on an average 0.16 norm. HCl. 
As NENCKI and StEBER already stated a short time ago, the 
pepsin is precipitated for the greater part from the pure gastric 
juice of the dog, in the same way as from the extract of the 
mucous membrane of the stomach, prepared with hydrochloric acid, 
as soon as the amount of acid is decreased to about 0.02 0/,: 
the best by dialysis. In the dialysator the substance is then 
deposited just like the pepsin out of the mucous membrane of the 
pig, in the shape of transparent small globules. While however 
the pepsin of the pig, at least when dried, was always somewhat 
coloured, even when it was separated out of a solution, in which 
no decided colour could be seen, the pepsin of the dog was always, 
) Zeitschr. f, Physiol. Chemie, Bd, XXXII, 8, 291, 
