( 418 ) 
always found to be very slight. In the pepsin precipitated by 
dialysis it was + 0.1°/,, in that which was first precipitated by 
ammonium sulfate += 0.20/,. 
Mme, SCHOUMOW —SIMANOWSKI !) found for the pepsin precipitated 
by cooling out of the gastric juice of the dog and washed with 
alcohol : 
C H 
50.71 TAT 
and for pepsin precipitated from the gastric juice, by saturating 
with ammoniumsulfate : 
50.37 6.88 14.55 
EEN baer 
where the amount of ash was not taken into account, whereas 
Nencki and SIEBER found, when analysing the substance precipitated 
by dialysis: 
C H N 5 
51.26 6.74 14,33 1.5 
and 
51.99 7.07 14.44 1.63 
is the average result of the four preparations analysed by me, of 
pepsin precipitated by dialysis. 
The difference concerns especially the amount of carbon, which was 
found by me in all my preparations higher than by the Russian 
investigators, though my figures are already very near those of 
NeNcKI and Sreper. On account of the absence of phosphorus in 
my preparations and also of the smaller amount of ash (N and S 
found 0.57°/)) I think I may consider the substance prepared by 
me, as being better purified. Also in the pepsin out of the mucous 
membrane of the pig’s stomach, which I did not succeed in libera- 
ting from phosphorus, I found a lower amount of carbon, varying 
between 48.18 and 50.77°/). 
In the ash I could detect iron in accordance with NENCKr and 
SreBER. I have not made determinations of the amount of iron. 
1) Arch. des Sc. biol. T. II, p. 463. 
