( 480 ) 
bacteria; hence a greater number of dark and evenly coloured 
organisms are microscopically detected than corresponds with the 
number of living bacteria. 
Perfectly healthy rabbits were killed by the blow on the neck ; 
directly after death the abdomen was opened, the bowels were 
bound off with sterilised silk threads and the contents of the different 
parts of the intestine were introduced into sterilised mortars under 
aseptic precautions. By means of sterilised pestles the substance was 
evenly mixed in the mortar; when making the dilutions the intes- 
tinal contents were for some time thoroughly shaken in sterile Hasks 
with porcelain balls. In particular for the contents of the small 
intestine which mostly consist of a viscid, sticky mass, in which the 
lower organisms are most unevenly distributed, such a laborious 
manipulation cannot be dispensed with in order to get a homoge- 
neous emulsion. Nearly always all the contents of each portion of 
the intestine were used for the research, with exception only of the 
Coecum, of which after previous emulsion, 10 grs. at least were used. 
In the different parts of the intestinal canal there now appeared 
to be a great disproportion between the number of cultivated and 
that of microscopically counted bacteria; the culture under other 
circumstances or on other nutrient media had no perceptible influence 
on the surplus of microscopically countable bacteria. 
TA Beli 
Parts of the intestine Times of the PURE chine | Increase | pee of bacteria | Increase 
ae Ce ee | found by culture in 
of rabbit n? 2. research. mee. del with | 1 mgr. of contents. with 
| directly 685.000 | | ct organism on 5 mgrs. | 
Small intestine........... | | 206.000 | 275. 600 
| 24hoursat 37°C. 891.000 275.000 | 
Lee alice 26.975.300 |, more than] 136 |, more than 
Coec. and Proc. vermiform. | E 121/, 
24hoursat37°C.;  39.792.000 millions 12.558 .700 millions 
| directly 8.565.000 nearly 67 nearly 
Large intestine and Rectum ladi 91/, 
‘24 hoursat37°C | 17.937.000 ) millions 9.432.000 millions 
