(135) 
lakmoid-paper as indicator. The quantity of Nag CO; appeared to 
range between 0.17 0/, and 0.24 0/,. | 
The proportion of the chlorine was determined by titration accord- 
ing to VOLHARD’s process. 10 CC of the juice were equal to 11.4 CC 
of decinormal silver nitrate solution and therefore contained a quantity 
of chlorine, such as is present in a 0.96°/, solution of sodium 
chloride. The total quantity of solid substances in the opalescent 
liquid amounted to 1.066 °/,. 
The freezingpoint-reduction, determined with BECKMANN’S apparatus, 
amounted to 0.620°. This is greater than that of normal human 
serum, which averages 0.57°. | 
II. Action of the intestinal juice. 
If we compare the results of Demant, TuBBy and MANNING and 
NAGANO concerning the action of the juice examined by them, it 
appears that all agree that its action on nutritive matter is insigni- 
ficant. Its action on proteids like fibrin and boiled white of egg is 
doubtful; so also is its action on cane sugar and fat; it sometimes 
has a feeble digestive action on starch. 
Investigations made on dogs during the last few years, in PAWLOW’s 
laboratory, have however cast a new light on the significance of 
the intestinal juice. It has been shown in these researches that the 
juice has little or no ability by itself to digest nutritive matter but 
that it possesses a powerful action when it codperates with pancreatic 
juice. According to PAwLow, the intestinal juice of the dog possesses 
the power of increasing considerably the action of the ferments of 
the pancreatic juice, and this is specially the case in regard to the 
proteolytic ferment, trypsin. We have therefore to deal with a ferment 
in the intestinal juice, which supports or increases the action of 
other ferments, „a ferment of the ferments”, which PAWLOow has 
named ,enterokinase”’ +). 
Our aim was now in the first place to ascertain, whether “entero- 
kinase’, found in dogs is also present in the intestinal juice of man 
and if this should be the case, to investigate further the nature of 
its action. 
The juice used in this work was freed from morphological elements 
by centrifugalizing. 
oe 
1) Pawrow. Das Experiment als zeitgemässe und einheitliche Methode medizinischer 
Forschung. Dargestellt am Beispiele der Verdauungslehre, Wiesbaden 1900, 8S. 15, 
