(7363 
We have already mentioned that this was opalescent. It is rendered 
as clear as water by filtration through a Chamberland bougie, but 
the filtered and unfiltered juice have the same actions. 
Let us begin by examining the 
a. Behaviour of intestinal juice in reference to the action of 
trypsin on white of egg. 
For these researches white of hen’s eggs was used, which accord- 
ing to Mrrrr’s method, was coagulated in narrow glass tubes by 
heating to 95°. The digestion was determined quantitatively by 
measuring on both sides the length of the little column which had 
disappeared. Two little tubes were used in each experiment and in 
the following tables the lengths of the four consumed little columns are 
added together. 
It was now seen that the intestinal juice alone had effected no 
visible change, after having been allowed to act on the tubes with 
coagulated white of egg for 48 hours or longer. 
Along with these experiments with intestinal juice, other experi- 
ments were performed in exactly the same way with a 1°/,-solution 
of trypsin (Merck) in a 0.3°/,-solution of Nay COs and at the same 
time with a trypsin-solution, to which intestinal juice was added. 
It was now evident that both the liquids containing trypsin had 
digested white of egg, the latter mixture however being by far the 
more powerful. 
Intestinal juice had therefore in this case exercised a furthering 
influence on the action of the trypsin in digesting white of egg. 
The following figures will make this clear. 
Furthering influence of intestinal juice on the digestive action of trypsin on 
white of egg. 
| Millimeters of white of egg consumed in 
| 24 hours. 2X 24 hours. 
2 
5 ce of the trypsin sol. +- 5 ce water....... | 4.50 | 9 
5 ce of the trypsin sol. + 5 ec Na, Co, 0.3 pCt. | 5) | 11 
5 ce of the trypsin sol + 5 ce intestinal zadel 7.50 20 
Intestinal juice alone....... SER - eee 2 0 | 0 
Not all commercial preparations of trypsin, however, gave such 
Pp Di ) ) o 
