( 738 ) 
| Millimeters of white of egg consumed 
After After After 
17 hours. 45 hours, 88 hours, 
2 drops of pancr. juice +} 3 cc. water.......... 0 0.7 7 
ZD De AD » +3 ec. NagCO,0.15 pCt. 0 0 10 
LD <a D » + » 0.3 » 0 0 42 
BB Pp » + » d5D 1D 0 0 0 
De Ne » + » 3 » 0 0 0 
Tubes empty, 
Danen eee » +  unboiled int. juice 2.5 11 therefore 24 at 
the least. 
De eS ow > + boiled » » 0 0 2 
There is no need to heat the intestinal juice to boiling to make 
it inactive ; the same result can be obtained by heating for 3 hours to 67°. 
In our next series of investigations, we ought to ascertain quan- 
titatively the power of intestinal juice in setting free trypsin from 
different quantities of zymogen. To this end, increasing quantities 
of fresh pancreatic juice were every time mixed with 5 ec. of 
intestinal juice, and the power of these mixtures to digest white of 
egg was examined. 
| Millimeters of white of egg consumed 
After After After After 
18 hours. | 41 hours. | 65 hours. | 99 hours. 
1 drop of pancreatic juice + 5 ec intest, juice 0 | 45 4 45 
2 drops —_ | Rig Sa) MEPA en Bie: 75 10 13 
Sn 5 pi Eloro boss 4 85 1 A43 20 
A veren » en Ee ax. G3 4 8.5 125 | 20 
Bae es LA re Ae ye 0 4 8.5 412.5 20 
These experiments show that a definite quantity of intestinal juice 
can only render a limited quantity of zymogen active for if 5 ce. 
intestinal juice are added to more than 2 drops of pancreatic juice, 
the digestive power is not thereby increased. 
This result does not confirm the opinion of PAwrLow and his 
collaborators, that the active substance of the intestinal juice is to 
be regarded as a ferment. If this were the case, then the digestive 
