(741 ) 
LINTWAREW’s work'), but the process of preparing it, and trans- 
ferring it into the glass tubes, was a difficult one. 
First the intestinal juice itself was examined. It appeared that 
in most cases this caused some digestive action, but this was extremely 
trifling compared with the powerful action which fresh pancreatic 
juice exerts. 
Room-temperature 
| 
| Dissolved after Cons. after 
| 18 hours. | 48 hours. 
Intestinal juiesc along. 407 Aetlare tuna 0 | 3 
Boiled inpests Sule. clave saters effe 0 1.50 
1 drop pancreatic juice + 2 cc. water 9 4 
In contrast with white of egg, starch is therefore immediately affected 
by fresh pancreatic juice; in other words, the diastatic ferment 
as such is already present in the pancreatic juice, which is not the 
case with the tryptic ferment. It might have been possible however 
that the pancreatic juice, used in these experiments, had already 
undergone a transformation for some cause or other, when the 
starch tubes were brought into contact with it. That this was not 
the case was proved from the fact that coagulated white of egg 
was not affected at all by the same juice. The question next arose 
whether intestinal juice has the power of increasing the activity of 
the diastatic ferment of the pancreatic juice, either by liberating an 
enzyme from a zymogen (in which form it might still be partly present) 
or by supporting the action, according to PawLow’s hypothesis. 
It was however found that it made no difference whether 2ce. 
water or 2cc. of intestinal juice are added to the pancreatic juice. 
The possibility still remained that the quantity of intestinal juice 
in relation to that of pancreatic juice, had been too large and that 
the surplus had acted as a diluent of the amylopsine of the pancreas, 
in the same way as it may do in relation to trypsin. 
Therefore mixtures of pancreatic juice were made, with relatively 
smaller quantities of intestinal juice, but the amylolytic power was 
never increased in any sample. 
d. Action on fat. 
Here the question had to be answered whether intestinal juice as 
such was able to split fat into fatty acid and glycerine, and further 
1) J. J. Lintwarew, Dissert. Petersburg 1901 (Russian). 
