114 K. A. :\1INC}I1N AND II. y\. WOODCOCK. 



on slides were fixed in osniic vapour^ for half a minute and 

 then placed in absolute alcohol for ten minutes or so. 'J'hey 

 were stained with (xiemsa's solution in the ordinary manner 

 for two and a half to three hours and afterwards differentiated 

 with orange-tannin and acetone. Smears on cover-slips, on 

 the other liand, were at once dropped face downwards into 

 sublimate-acetic mixture or Scliaudinn's fluid for fixation, 

 after which they were brought up to 90 per cent, alcoliol. 

 These smears were stained either with iron-haematoxylin or 

 with Twort's stain and finally mounted in balsam. Fuller 

 details regarding this mode of procedure will be found in 

 Mincliin (11), the method there described being that followed 

 in the present case. The figures of the luvmogregarine are 

 all drawn to a mngnification of 3000, those oF the trypano- 

 somes to a magnification of 2000. We are indebted to Miss 

 Rhodes for drawing the greater number of the figures and for 

 colouring nearly all, and take this opportunity of expressing 

 our thanks to her. 



I. H.F.MOGREGARINA ROVIGNENSIS, N, SP. 



(Figs. 1-28, 39-50, and 58-62.) 



This parasite was found on three occasions in the early 

 spring. In the three gurnards in which it was seen it was 

 not nt all infrequent in the blood, as we have since learnt by 

 the study of stained preparations. The parasite, chiefly in its 

 small intra-cellular form, was observed in the living condition 

 in cover-slip preparations of fresh drops of blood, but only 

 with difficulty, since it was not readily distinguishable from 

 the cytoplasm of the blood-corpuscle. When made out with 

 certainty it was seen as a small oval area, somewhat clearer 

 and paler than the surrounding cytoplasm. Xo prominent 

 refractile granules were noticed, nor could the nucleus be 

 made out satisfactorily. We did not observe any alteration 



' A 4 per cent. sohTtion of osmic acid was used, to which a drop or 

 two of acetic acid had been added. 



