GANTMEDES ANASI'IDIS (nOV. GEN., NOV. SP.). 167 



The nucleolus thus seems obviously to be the chief agent 

 concerned in the manufacture of food-stuifs (for theories 

 regarding the action of Mann's methyl blue eosin see Leger 

 and Duboscq (2) ). 



What is the function of the rest of the nucleus in this 

 period remains uncertain, though its large size shows that 

 it must play some important part in metabolism. The chief 

 interest here lies in the behaviour of the nucleolus, which 

 migrates out to enter into direct relations with the cytoplasm 

 at the beginning of the second trophic period, when assimila- 

 tion begins to be greatest, and at its close, when all 



Text-fig. 4. 



Section of one of the niiclei of a conple in the second trophic 

 phase. The niicleolus does not project very far, and the 

 siu'face of the absorptive area is flnsh with that of the 

 niicleolvis, althoiigh tlie area itself is very large. 



assimilation ceases, returns, as will be seen later, to the 

 interior of the nucleus. 



(iii) Encysted Phase. 



The cyst-wall, though always fairly strong, varies a good 

 deal in thickness. It stains bright blue by Mann's method, 

 bright red with carmine, but not strongly with hsematoxylin. 

 From it often project radially inwards curious irregulai-, 

 branching filaments, never reaching much more than a third 

 of the way to the centre, as to whose nature and function I 

 am quite in the dark (fig. 16). 



The cytoplasm is reticular, with minute granules on the 



