254 (;. M. WKN'voN. 



dried tilras as an irregular clinnp of red staining granules. 

 In other words, its appearances are like those of the nuclei of 

 trypanosonies in similarly prepared films. In films fixed and 

 stained by the wet method described above the structure of 

 the nucleus is comparable with the appearances to be made 

 out in the living organisms. 



The details of longitudinal division can be followed in the 



Tkxt-figs. 12, 13. 



.^. 





/ 



I 





13. 



Dividing' forms. 



stained preparations. The large karyosome becomes elongated 

 and constricted, and finally divided into two parts (fig. 15). 

 I was never able to detect within the k.'ir3'OSonie a centriole, 

 spindle, and lequitorial plate, as described by Berliner in the 

 division of Copromouas major, but the division takes place 

 in an amitotic manner, resembling that of Copromonas- 

 sub til is (Dobell). Most usually the karyosome becomes 

 distinctly dumb-bell shaped as in fig. 12, but at other times 

 the division is along the longitudinal axis of the elongated 

 karyosome, the resulting daughter-kai'vosomes each being 

 elongated (figs. 15, 16). Following the division of the karyo- 

 some the nuclear membrane elongates while the daughter- 



