SOME OBSERVATIONS ON A NEW GREGARINE. 273 



it is only on very thorough differentiation that the chromo- 

 somes are rendered visible. The spindle fibres appear to 

 merge with the terminal chromatin mass. Distal to this there 

 is no true astral arrangement visible. 



Each terminal chi-omatic aggregation now gives place to 

 a definite vesicular structure, situated at the poles of the 

 spindle and forming the centre of a definite astral radiation 

 (figs. 14 and 15). Simultaneously with the appearance of the 

 vesicle, the chromatin streaks and granules disappear from 

 the spindle, so that the more definite the terminal vesicle, the 

 fewer the chromosomes on the spindle. Fig. 12 shows a ring- 

 like arrangement of the terminal chromatin aggregation at 

 one pole of the spindle (a), while fig. 15 shows a true polar 

 vesicle containing definite granules of chromatin, in one 

 instance arranged indiscriminately around the circumference, 

 in the other accumulated at one point upon it. These vesicles 

 are the points upon which the very definite spindle-fibres 

 converge, and measure from l|-2j/.t acj-oss. In figs. 14 and 

 15 it will be noticed, firstly, that — apart from the granules 

 within the vesicles and the karyosome products — thei"e are 

 practically no other discrete chromatin elements to be seen ; 

 secondly, that some of the spindle-fibres plainly run down 

 into the midst of the nuclear area and the karyosome remnants, 

 where these latter are not already lying on the spindle. In fig. 

 15 will be seen, lying close to the large irregular karyosome 

 residue, a collection of deeply stained granules, which are 

 connected with the karyosome and with each other by deeply 

 stained strands. They have probably been recently thrown 

 out from the karyosome, which is much distorted from its 

 original spherical shape. 



The latest stage of the first division represented among my 

 slides was unfortunately injured before anything more than a 

 rough drawing had been made of its structure (fig. 16). It 

 represented the spindle very much drawn out, just before the 

 final separation of the two daughter-nuclei. There was at 

 each pole a well-marked vesicle, containing numerous granules 

 of chromatin, and distal to this vesicle was a mass of achro- 



