ARTIFICIAL CULTURE OF MARINE PLANKTON ORGANISMS. 361 



On the Artificial Culture of Marine Plankton 



Organisms. 



By 



E. J. Allen, O.Sc, 



Director of Lalwratories and Secretary of the Marine Biological 



Association ; 



and 



E. ^V. INelsoiV 



Assistant Naturalist. 



Contents. 



PAGE 



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368 



Introduction . . • • 



I. Culture of Plankton Diatoms 



A. Practical Culture Methods 



1. Miquel's Method . 



2. Houghton Gill's Method 



3. (a) Modification of Miquel's Methods : " Miquel 



Sea-water " . 



(b) English Channel Water . 



(c) Tank-Water 



(d) Animal Charcoal Water . 



(e) Peroxide of Hydrogen Water 



(f) Cultures in these Media . 



B. Experiments with a view to Determining the Con- 



ditions which underlie the Successful Culture of 

 Diatoms . . . • 



Methods . . ■ • 



The Sea-water employed 

 The Constituents of Miquel's Solutions . 

 Animal Charcoal and Peroxide of Hydrogen 

 Reviving Exhausted Cultures . 

 Silica . . • • 



Organic Infiisions 

 1 Owing to pressure on our space, this memoir could not l^e published 

 when first in type. It has in the meantime been issued in the ' Journal 

 of the Marine Biological Association,' vol. viii, No. 5.— E. R. L. 



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