388 E. J. A[J,KN AND K. \V. NKI.SON. 



under observation for at least four months. Series were 

 made as uniformly as possible, and controls in standard 

 media were included in each. The strength of the various 

 solutions used in these experiments was the same as in 

 Miquel's formula. 



A. Outside water + solution A + solution ]5 (normal Miquel 



sea-water. 

 First control. 



B. Outside water + solution A + Na;2HP0t solution + 



FeCl.j solution + CaCL solution. 

 Second control. 

 Good normal cultures were alwavs obtained in these two 

 controls. 



C. Outside water + solution A + Na.iHl^Oj solution. 



A very uncertain medium. Sometimes no growth has 

 been recorded, and at other times a fair growth 

 results, but these cultures are never equal to normal 

 Miquel. 



D. Outside water + solution A + FeCl. solution. 



Occasionally a very small growth has been obtained, 

 but at the best it is very poor. 



E. Outside water + solution A + CaCL solution. 



About equal to D. 



F. Outside water + solution A + NaoHPOi solution + 



FeCla solution. 

 Uncertain as c. No cultures have been obtained equal 

 to the best in c. 



G. Outside water + solution A -1-NaoHPOi solution + CaCl, 



solution. 

 Some cultures very nearly equal to the controls have 

 been obtained in this medium. 

 H. Outside water + solution A + FeCl.s solution + CaCU 

 solution. 

 Poor, about equal to D. 

 Analysing the above results we see that — 

 (1) None of these modifications of solution B give results 

 equal to solution B itself. 



