OBSEEYATIONS ON TRYPANOPLASMA CONGERI. 489 



kinetonucleus are situated the two minute blepharoplasts, 

 from Avliich the tlagella arise. I believe tliem to be always 

 two in number, but in iron-hgematoxylin preparations they 

 are so minute and often so close together that it is impossible 

 to resolve tliem as two granules, and they may appear as a 

 single dot." 



In the active Trypanoplasma congeri the two flagella 

 always appear to me to arise from a single basal granule, and 

 from what I have seen of the dividing and resting forms I am 

 certain that if the flagella do not arise from a single basal 

 granule, the connection between the two granules must be so 

 intimate that the flagella always behave as though they arose 

 from a single point. Passing down the side of the animal 

 under the membrane a row of very faintly staining rounded 

 granules are frequently seen; these may correspond to the 

 structures described in Trypanophis, or possibly to the far 

 more strongly staining granules seen in some forms of 

 Trichomonas, l^he trophonucleus in the elongate form of 

 Trypanoplasma congeri lies about one third of the 

 animal's length from the anterior end, and usually consists 

 of a conspicuous membrane containing a darkly staining 

 elliptical karyosome, which is usually surmounted at its 

 anterior end by a cap of chromatin granules. In some cases, 

 however, the karyosome is central and the granules are 

 arranged round it. These appearances recall Schaudinn's 

 figure of the chromosomes in the resting nucleus of Try pan o- 

 morpha and Leger's description of the chromosomes of 

 Trypanoplasma in testinali s. It will, however, I think, 

 become abundantly clear from the behaviour of the dividing 

 trophonucleus described below that it is impossible to regard 

 the chromatin gianules of Trypanoplasma congeri as 

 chromosomes. 



The kinetonucleus is usually a very darkly staining carrot- 

 shaped structure lying laterally near the animal's anterior 

 end, the narrow posterior end of the kinetonucleus passing 

 down the animal's body to end in the region of the tropho- 

 nucleus. In some cases the kinetonucleus presents an almost 



