490 C. II. MAliTIX. 



segmented appearance, and apparently this appearance has 

 in many cases been taken as an early indication of division, 

 though T believe this interpretation to be erroneous. 



Division. 



All of the preparations here figured are taken from films of 

 the stomach of a fasting conger which had been kept in the 

 tanks at Millport for four months, and was killed at 5.30 p.m., 

 November 27th. In the early stage of division (PI. 21, fig. 2) 

 the body of the animal becomes slightly shorter and thicker. 

 The basal granule of the flagella divides, and this is followed 

 by a splitting, first of the anterior Hagellutn along its whole 

 length, and then of the posterior flagellum with its membrane. 

 The trophonucleus and its contained karyosome become 

 larger, and I believe that the intra-nuclear chromatin granules 

 (? the " chromosomes" of Schaudinn) at this stage become 

 condensed on to the karyosome. The kinetonucleus at this 

 stage becomes slightly thicker, but sliows no distinct indica- 

 tion of division. In the next stage (PI. 21, fig. o) the flagella 

 have split along their whole length, and it is important to note 

 that, in marked distinction to the state of affairs found by 

 Friedrich in Trypanoplasma helicis, I have never been 

 able to find the slightest evidence of the growth of new 

 flagella in any stage of division. The trophonucleus now 

 assumes a spindle shape, and tlio karyosome divides; the two 

 halves, however, remaining connected l)y a rod, which persists 

 until a very late stage of division. It might have been 

 expected that some sign of the so-called chromosomes would 

 be found at this stage lying around the dumb-bell-shaped 

 karyosome in the spindle-shaped nucleus, but no trace of them 

 has been detected. It is, of course, possible that this may be 

 due to faulty technique, but so many of these dividing stages 

 have been found lying near resting forms with nuclei clearly 

 showino- these o-ranules that I believe this hypothesis is 

 untenable. The relation of the axis of the trophonucleus 



