662 H. M. WOODCOCK. 



usual manner. Many smears of the blood Avere made, some 

 at once, others after waiting a moment or two, and with or 

 without the addition of a drop of salt-citrate solution. 



The bird was again examined on the afternoon of June 

 22nd, when one trypanosome was seen in two cover slip pre- 

 parations after some searching. Another night examination 

 was made about 1 a.m. on June 3Uth. Compared with the 

 previous night examination there appeared to be as many 

 trypanosomes present, but the mature Haltei-idia did not 

 seem to be quite so numerous as befoi'e. After a similar 

 procedure I at length killed the chaffinch (about 2.30 a.m.) 

 in order to obtain smears from the internal organs — heart, 

 liver, spleen, bone-marrow, kidneys, etc. Most unfortunately, 

 I omitted to make any preparations from the lungs — an over- 

 sight wliicli I have since greatly regretted. I need only 

 mention here that the trypanosomes were afterwards found to 

 be comparatively few in number in preparations from the 

 bone-marrow, while in smears from the liver, etc., they are 

 very scarce. As regards the peripheral circulation, the 

 parasites are certainly more numerous in these night-slides 

 than they are in those taken (from the same situation) in the 

 daytime (afternoon). Hence thei-e would seem to be, to 

 some extent, a wandering of the trypanosomes from the 

 internal organs (probably chiefly from the bone-marrow, 

 which is their principal " internal " habitat) into the peri- 

 pheral circulation during the night-time. 



Halteridiuui in Relation to the Corpuscles. — As 

 already indicated, the Halteridial infection of this bird was a 

 very strong one, and the parasites were very numerous at 

 this time; in fact, in some smears, for instance, from the 

 livei", they are almost abundant. The Halteridia are of all 

 sizes, from minute forms up to fully grown adults. Xearly 

 all the parasites are intra-cellular. Until recently the only 

 cases in which I observed any forms free from the corpuscle^ 



' Of course, ripe sexual individuals, which have become roiuided off 

 and liberated themselves from the corpuscles, are not included in this 

 statement ; neither are distorted or irregular individuals, which have 



