730 H. M. WOOJJCOCK. 



Schaudinn considered that these were simply the resting- 

 phases of large, sexual trypanosomes, which had come into 

 relation, in a peculiar manner, with the leucocytes, causing 

 the host-cell to become greatly extended and altered in form. 

 I agree with Wenyon that this view cannot be sustained. 



Structure of Gametocy tes. — In stained prepara- 

 tions the parasites occur in two well-marked and distinct 

 forms, which represent without doubt male and female game- 

 tocytes, since they agree very well with these types in other 

 leucocytozoa. The parasites occur in all sizes, from very 

 young forms up to what are probably fully grown, mature 

 individuals (tigs. 19, 20, 24, and 25). Even in fairly young 

 individuals the male or female character can be often recojf- 

 nised (figs. 19 and 24). The diameter of a rounded individual 

 averages about 8^ to 9 ^ ; the ovoid parasite of fig. 26 is 11 fx 

 by 6|^ fi. Female forms appear to attain a slightly larger 

 size than male forms. 



Comparing a male gametocyte with an individual of female 

 sex, the cytoplasm of the former stains much paler than that 

 of the latter, and appears to be more homogeneous in structure. 

 The cytoplasm of a female individual is distinctly granular. 

 The nucleus of a male form is large and somewhat diffuse ; 

 it appears to contain a number of small chi'omatin granules 

 (probably really chromatin " dust," which stain pinkish. 

 The female nucleus is small, compact and dense; its chromatin 

 grains stain darker and more intensely than in the other case. 

 Both in the male, as well as in the female form, a definite 

 small chromatic body is sometimes found outside, but close 

 to the chief nucleus (figs. 20, 25, and 26) ; it has also been 

 seen in small parasites (figs. 19 and 60). This small body 

 corresponds to that associated with the nucleus of L. 

 ziemanni, where it was first described by Schaudinn. As 

 I hope to have something to say subsequently, in conjunction 

 with Professor Minchin, upon the nuclear structure of the 

 latter parasite, I will not discuss this point at present, 

 especially as my material is limited and all stained by 

 the Komanowsky method. 



