7r>6 MIJKIEI. KOr.KUT.SON. 



iiru ouly instances of (perhaps precocious) association. After 

 a time (figs, 38-41) the haemogregarines penetrate the intes- 

 tinal wall, where appearances quite different from those just 

 described suggest conjugation of a type closely resembling 

 that found by Siedlecki (14) in Adelea ovata, and by 

 Perez (10) in Adelea rnesnili. Fig. 44 gives a picture of 

 an early stiige ; the macrogameta has become differentiated 

 as a large rounded organism with a nucleus in which the 

 chromatin is beginning to form a ratiier diffuse mass instead 

 of being arranged in the definite granules seen in the motile 

 phase. The nucleus of the microgametocyte is very com- 

 pact, and stains deeply, the protoplasm has not fused with 

 that of the microgamete, nor does it appear to do so sub- 

 sequently. 



From appearances such as fig. 45, the microgametocyte 

 nucleus seems to divide into three or four, of which two or 

 three, as the case may be, remain outside and degenerate; 

 they sometimes persist for a long time^ and are to be seen 

 forming a dense mass of chromatin on the edge of the 

 sporocyst (see figs. 49, 51, 52). The division of the nucleus 

 of the microgametocyte into four is probably the more normal 

 condition, the cases where three are formed being most likely 

 due to the suppression of one of the divisions. One of the 

 four microgamete nuclei thus formed appears to pass into 

 the protoplasm of the macrogamete ; unfortunately quite 

 clear pictures of the fusion of the gamete nuclei and the first 

 division of the zygote nucleus were not found. Fig. 47 B 

 shows a condition su^-gestinof the latter stage, but in view of 

 certain reactions on the part of the host-cell to be noted 

 later, I do not feel perfect confidence in this interpretation. 



It is quite impossible to pass over these appearances without 

 noting their very probable significance as conjugation and 

 their close resemblance to the fertilisation of Adelea ; at the 

 same time I am fully aware of important gaps in the series. 

 Great caution is required in interpreting these appearances, 

 as degenerating hasmogregarines are occasionally found in 

 the gut wall. Moreover, the host-cell seems sometimes in 



