fill 



of a rabbit at the inside of (he thigh. These extracts also contained 

 traces of turpentine. 



Similar tubes were placed at the other hind leg with the same 

 contents, but without turpentine. 



After 20 hours the leucocyte columns, which owing to cliemotaxis 

 had entered the tubes, were measured. This rendered it possible to 

 establish if, and if so, to what extent, turpentine had promoted 

 chemotaxis and stimulated the phagocytes. 



The second method consisted in 0.3 cc. of turpentine being injected 

 under the breast skin of some rabbits ; it was then determined if a 

 greater amount of leucocytes had entered the capillary tubes with 

 coli-extract, than if the same rabbits had been injected with 0.3 cc. 

 of NaCl-solution instead of 0.3 cc. of turpentine. 



Before stating the results obtained on the effect of turpentine we 

 shall communicate a series of experiments which were made to 

 ascertain the degree of accuracy of the method. In four rabbits 

 capillary tubes are placed right and left under the skin of the leg. 

 These tubes are filled with the same extract of coli-bacteria in NaCl 

 0,9 7„. 



The following table will require no further explanation. 



TABLE I. 



Effect of extract of Coli-bacteria on chemotaxis. 



Left leg. Rigtit leg. 



Extract of Colibac- Extract of Coli bac- 

 teria ill 0.9% NaCl teria in O.QOo NaCl 



Rabbit 1 



„ 2 



» 3 



4 



Total of 4 leucocyte- 

 columns after 



2 hours 



4. 1 mm 



6.3 „ 

 5.6 „ 

 5.6 .. 



4.5 mm 



5.5 „ 

 5.5 „ 

 5.2 „ 



+ 0.4 



— 0.8 



— 0.1 



— 0.4 



This table shows that the greatest deviation amounts to 0.8, whilst 

 the deviation in all 4 rabbits together only comes to 0,9 mm. 



First method. 



Under these circumstances it could be established now, to what 

 extent an addition of turpentine to an extract of B. Coli in NaCl- 

 solution would affect the degree of chemotaxis. ^) 



1) The technical details were about the same as those we described in Virchow's 

 Archiv B. CLVI p. 821), 1899 and in "Physik. Chemische Untersuchungcn über 

 Pliagozylen". Bergmann 1912. p. 9i foil. Only instead of coi'k paiaffin was used 

 to keep the capillary tubes in their places. 



