fil3 



TABLE III. 

 Effect of coli-bacteria-extract on chemotaxis. 



Left lee ^'S^t leg. 



iExtractofcoliLcteria,E^'7„^*°^QS°l';j!f^le"^ 



in OQiV. Nan '" ^'^ ^^^' 



I inU.y/oNaU +1:500.000 turpentine 



As we observed before, the second method of investigation consist- 

 ed in turpentine being injected subcutaneously in the lower chest 

 to enable it to spread through the body with the blood, thus entering 

 also into the Ij-mph of the hind leg, where tubes with extracts of 

 bacteria in NaCl 0,9 "/o ^^^id been placed. The experiment showed 

 that turpentine entered the system but slowly. For when after 7 

 days the injectionplace was opened, the mucous mass which came 

 out still strongly smelled of turpentine. Turpentine, indeed, does not 

 dissolve readily in watery fluids. 



Since in different rabbits the chemotactical action is not (he same, 

 the columns of eacli rabbit were measured without anything bein^- 

 injected. When this had been determined half the rabbits were 

 injected under the breast with 0.3 ccm. of turpentine and the other 

 half, as a test, with 0.3 ccm. of NaCl-solutiou 0.97u- 



Five or six hours after injection the capillary tubes were placed 

 under the skin and they were removed after 20 hours. The following 

 table gives a survey of the results obtained. 



Now if w^e compare the total of the differences of 2, 4, 6, 8 and 

 10 which comes to -}- 6,1 mm. with the total of the differences of 

 1,3,5,7 and 9, which amounts to — 0,1, then if appears that the 

 su/jciitaneous injection of turpentine has evidently stimidated the 

 chemotaxis. 



It must therefore be concluded tiiat, in accordance with our hypo- 



