95.^ 



made experimenls in wliich I removed, a? much as possible, incal- 

 culaUle influences. 1 |)rO('ceded in the foilowiuij,- maimer: 



A short time before I hnng the iV(\i>- in tlic damp ease, or occasion- 

 ally a tew davs previously, I cut, on the right side, the Rami com- 

 mnnicantes and the right sympathetic cliain al the top. By binding 

 up the heart I killed the frog, then I passed a thread through the 

 two jaws, and on these I hung the frog in a closed, glass-case that 

 was kept damp by a wet sponge and by thoroughly wet filter-paper 

 on the bottom. The damp case was then placed in a room in which 

 the temperature was raised as high as 30° to 35° Celsius. The frog 

 was consecjuently placed in regularly heated damp surroundings. 

 By this higher temperature the process of rigor mortis is consider- 

 ably shortened. It could easily be observed in these frogs, that the 

 right hind-leg hung down suppler than the left one, that consequently 

 the tonus had disappeared from the 'muscles at the right side. 



A short extract from the protocols may follow here: 



I. 17 January. All Rami communicantes of the right side are cut. 



20 January: After the heart, has been bound up the frog is hung in 

 the damp case. 



4.30. Left hind-leg is drawn up, stiffness in hip, knee-joint and foot. 

 The left elbow-joint is sliifencd, no dillerence is to be observed in the two 

 shoulder-joints 



Bight elbow and wliole right hind leg are slill supple. 



5.30. Silnalion slill the same. 



9.30. The two liind-iegs are hanging in sliifencd tense and -dxliirlion 

 position; the two fore-legs stiffened in flexed position. 

 22 January, lligor mortis has ceased. 

 11. 21 Janua/ry. J. 15. The Rr. communicantes are cut through. 



1.30. The heart is bound up. The frog is hnng in the case. 



5.30. Left shoulder-joint is stiff, other joints are still sup|)le. 



7.30. Situation unchanged, 



8.30. Beginning of rigor moi'iis of the left hip. 



9. Bight shoulder b?gins to become stiff. 



10. Left elbow stiffened. 



11.30. Left knee begins to stiffen, the hip is quite stiff. 



22 January. Stilfened tense and abduction position in all joints of the 

 hind-legs. 



23 January. 9 a. m. The rigor mortis has ceased. 



111. 19 January. 11.15 a. m. The Br. communicantes are cut through. 



11.30. The frog is hung in the case after the heart has been bound up 



4. p. m. Left fore-leg stilfened in the elbow- and shoulder-joints, left 

 hind-leg is strongly Hexed and stilfened in the hip, doisal llexion and rigor 

 mortis of the foot-joint. 



4.30. Left knee is becoming stiff. 



5. p. m. Right elbow-joint, knee and hip begin to become stiff. 



62 

 Proceedings Royal Acad. Amsterdam. Vol. XVI. 



