485 



Therefore the calculation in the case of (1) gives a quantity of 

 diastase of 75,0, in (2) 47.6 and in (3) J 2.9. If it is assumed, that 

 in flasks 1 and 2 the same quantity of enzyme was present in the 

 culture-fluid as in flask 8, then there remains ('or the enzyme 

 in the mycelium for (J) 62, and for (2j 34.7. These tigures appear 

 to diverge widely, but nevertheless we shall soon show, that Iheie 

 is a great regularity in the quantity of enzyme found on daily 

 investigation. 



The experiment which is here described in detail was the be- 

 ginning of a whole series of determinations. Altogether 42 simikir 

 flasks were inoculated with Aspergillus, and after a given number 

 of days three of them were each time examined in the manner 

 described. The result is given in the appended table. In column I 

 the number of days is given, during which the culture lasted, in 

 column 11 the quantity of enzyme in the mycelium and culture- 

 fluid together, in column 111 the same for the other flask, in 

 column IV the mean of 11 and 111, in column V the amount of 

 enzyme in the culture-fluid alone of the third flask, in column VI 

 the difference between . IV and V, and finally in column VII the 

 dry weight of the crop of fungus in flask 3. Wherever the sign <^ 

 is found in the table, this indicates that the last test-tube still gave 



