486 



a reaction with the iodine-solution, so that the limit of hydrolysis 

 had not yet been reached. Wherever "trace" is written, the anaount 

 is less than 0.5, although a change in reaction was still cleai'ly 

 observable. 



The figures of columns II and III appear at first sight to diverge 

 widely, but on nearer inspection a certain regulai' course is however 

 noticeable, by which column IV is justified. The amount of enzyme 

 in the culture-fluid is generally very small, so thai the course of 

 column VI is scarcely different from that of column IV. There are 

 a few exceptions to this, but I think it t)etter to give (he complete 

 observations first and then only to f)egin a discussion of the figures 

 found. 



A second series of figures had reference to a number of cultures 

 which had been made at the same time as those already described, 

 and which oidy differed from them in that instead of 75, 150 cc. 

 of culture-fluid was used and in larger flasks, in consequence of 

 which the surface of the culture-fluid amounted to 47 square cm. 

 instead of 24 as in the foi-egoing series. There was indeed the possibility 

 that on this account the development ofJ.s/>^;y////?^9 might continue longer. 



„ 19 



,1 23 



. 26 



u 30 



„ 33 



. 37 



11.0 

 1.7 

 4.0 

 4.2 

 11.3 

 44.1 

 4.5 



1.2 

 0.0 

 0.0 

 0.0 

 0.0 

 0.0 

 0.0 



