487 



and with it also the formation of enzyme. Of these flasks half were 

 directly examined as to enzyme, the others after tliey. had stood foi- 

 an hour with tlie finely ground fungus-mass and had then been 

 filtered. The results obtained are shown in the fore-going table. 



In order also to trace during a longer period the course of diastase 

 formation, a new experiment was begun with 99 flasks each con- 

 taining 300 c.c. of nutrient solution and treated exactly in the same 

 way as the first series with this one ditference, that this time account 

 was taken of the (piantity of liquid which remained when cultivation 

 was over. In consequence of consumption by the fungus and of evapo- 

 ration this quantity diminishes, a fact that in the above experiment of 

 37 days could not have so strong an influence on the results as in 

 those lasting no less than 149 days. In consequence of this the 

 calculation was more complicated, but still sufficiently simple, and 

 after what has already been said, it will not be necessary to give 

 the slightly modified calculation here; we may only lemark that 

 the figure of column VI is therefore not exactly the difference 

 between IV and V. With this qualification the different columns in 

 the following table, have the same significance as in the first table 

 already given. 



The figures of column IV aie represented graphically in fig. 1 . In 

 this figure the age of the culture (in days) is measured on the axis of 



4 3 9 li 16(9 iJJl 30 33 jrvO*»W J. 5» 65 fi t9 et 33 lOO lOT IIV Itl UB IM l*i Hi 



Fig. 1. 



the abscissae, whilst the amount of diastase is respresented by the 

 ordinates. In figure 2 the figures of column VI are represented in 

 the same way. Valid objections may perhaps be raised against the 

 latter figures, because in these cases the amount of enzyme was 

 deducted, which occurred in the culture-fluid of a culture distinct 



