549 



CH,OH CH,OH CH,OH CH,OH 



H—C— OH H— C— OH H— (^— OH H— C— OH 



H_C— OH H— C— OH OH-C-H H— C— OH 



OH— C— H OH-C-H OH— C— H OH— C-H 



I I I ■ I 



H_C_OH C=0 H— C— OH OH— C— H 



I I I I 



C— OH CH,OH C—OH C-OH 



d-Glucose Fructose d-Galactose d-Maiinose 



(laevulose) 



The experiments were made in exactly the same way as was 

 described in tl)e articles cited above. The perfusion liquid was of 

 the following composition : NaCi 0,5 »/„, NaHCO, 0,285 «/o. KCl 0,01 7,, 

 CaCi, 0,2 "/„. This solution was prepared by mixing 



50 ccm. NaCl 10 V„ 



50 ccm. NaHCO, 5,7 V.. 



10 ccm. KCl 1 7, and 



40 ccm. CaCl, 5 7, 

 and adding boiled distilled water up to 1 Liter. Cei'tain quantities 

 of the sugars ^) were dissolved in this solution, but still the reduc- 

 tive capability was estimated before eacli experiment. This was done 

 in view of the possible errors in weighing oi' unknown differences 

 in the amount of water contained in the sugars. Bang's method (1916) 

 was used for estimating the reductive capability of the perfusion 

 liquid as well as that of the urine excreted. The reductive power of 

 the various sugars was expressed in the percentage of glucose contnuied. 

 As is well know the final titration is an estimation of Iodine 

 with the aid of amylum; the amount of glucose contained is then 

 computed from the quantity of Iodine necessary, by means of the 

 formula {a — 0,12) : 4, in which "a" is the number of c.c. solution 

 of Iodine used. 



A. Laevulose (Fructose). 



Experiment 1 (July 11, 1918) 



The perfusion liquid contains 0,1 "/o laevulose. 



The reduction, expressed in glucose, amounts to 0,21 %. 



0,1 ccm. urine from the right kidney needs 0,87 ccm. Iodine solution, which 



0,87-0,12 



corresponds to = 0.0875'Vo glucose. 



4 



1) We are indebted to Jhr. W. Alberda van Ekenstein, Diroctor of the laboratory 

 of the ministry of b'inan e and by Prof. H. J. Backer, for several of the sugars. 



3H* 



