( 614 ) 
For comparison the fibrinogen from 8 cem. of that part of the 
fibrinogen solution which had not been prepared with NaFl, was in 
the same way first coagulated and afterwards the fibringlobulin in 
the filtrate; although the fibrinogen solution used for this experiment 
contained 0.565°/, fibrinogen and so had been but little more con- 
centrated than the solution prepared with Na FI the quantity fibrin- 
elobulin found was remarkably larger, as there was formed abundance 
of floeculent precipitate by heating to 70°. 
Another experiment gave the following results. 
II. The solution of the fibrinogen not precipitated with Na Fl con- 
tained 0,634°/, fibrinogen, that of the fibrinogen precipitated with 
Na Fl 0,452°/,, after this the fibrinogen being removed from the two 
solutions by heating to 55—58° and by filtering off of the coagulum, 
5 eem. of each of the filtrates were mixed with 1'/, cem. of a 
saturated solution of picric acid. In the filtrate of the fibrinogen treated 
with Na Fl there was formed only an opalescence which after some 
time passed into a very slight precipitate; in the filtrate of the fibri- 
nogen not precipitated with Na Fl there was immediately a con- 
siderable flocculent precipitate. 
Ill. A solution of oxenfibrinogen was precipitated by four times 
the volume of a saturated natrium fluoride solution; afterwards the 
precipitate was centrifugalised off, washed with water and dissolved in 
4°/, salt; the solution contained 0,232°/, fibrinogen; after its having 
been removed by heating to 55—58° and filtering off of the coagulum 
the filtrate remained perfectly clear on being boiled, and so contained 
no fibringlobulin, although the original fibrinogensolution had been 
precipitated with Na Fi only once. 
So it appears that by means of natrium fluoride fibrinogensolutions 
may be obtained, which by heating produce no fibringlobulin or 
only traces of it. 
This confirms the opinion that the fibringlobulin was present 
already in the original, not heated fibrinogensolution either combined 
with fibrinogen or simply as admixture, and that consequently fibrin- 
globulin is not formed by alteration of the fibrinogen during the 
heating ; in the last case it could not be explained why the fibrinogen, 
prepared with Na Fl should not be altered as well in the same degree 
by heating in fibringlobulin. If however the fibringlobulin was present 
already in the fibrinogensolution, every thing may be explained in 
this way that on being treated with Na FI the fibringlobulin passes 
into the filtrate at any rate for the greater part, while the fibrinogen 
proper precipitates. The possibility that the fibringlobulin does not 
precipitate also appears from the following experiment. In a solution 
