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of horsefibrinogen prepared after the method of HAMMARSTEN the 
fibrinogen was coagulated by heating to 60° and filtered off; to the 
filtrate was added a double volume of saturated natrium fluoride 
solution; the liquid remained perfectly clear. 
The question whether the fibringlobulin passes into the filtrate 
when the fibrinogen is precipitated with Na Fl cannot be answered 
immediately by examining the filtrate, while the fibrinogen with 
Na Fl does not precipitate completely, so a certain quantity of fibri- 
nogen exists still in the filtrate, and when, after heating, fibringlobulin 
is still found, the possibility exists, that all this fibringlobulin proceeds 
from the quantity of fibrinogen present in the filtrate; only the quan- 
titative research can decide here; if on precipitating with Na Fl the 
fibringlobulin passes into the filtrate it must be possible to prepare 
from this filtrate nearly as much fibringlobulin as from the original 
fibrinogensolution. As the fibrinogen precipitated with Na FI is not 
perfectly free from fibringlobulin, an accurate agreement is not to be 
expected. In the first place I subjoin the results of such an experiment. 
a) 100 eem. of a pure horsefibrinogensolution, prepared after 
HamMarsten’s method were precipitated with 200 ccm. saturated 
natrium fiuoride solution. The precipitate was taken with a glass rod 
out of the liquid, pressed out firmly, dried to constant weight and 
weighed, the substance was burnt carefully, the weight of the ashfree 
substance proved to be 0,2485 gram. After the precipitate obtained 
with Na Fl had been removed a clear liquid remained, which was 
neutralised with some drops of diluted acetic acid, as the reaction 
of the solution of NaFl used was faintly alkaline, which mostly is the 
case. The liquid (285 eem.) was heated afterwards for a quarter of 
an hour in a waterbath to 55—60°; the coagulated fibrinogen was 
filtered off on a weighed, ashfree filter, with a diluted saltsolution 
and after that washed with water, dried to constant weight- and 
weighed together with the filter; the filter and the substance was 
carefully burnt. It proved, that 0.2262 gram ashfree fibrinogen had 
been present on the filter; this quantity was obtained from 285 eem; 
so in the original 300 eem. there would have been found 0.2381 gram. 
In order to determine the quantity of fibringlobulin 250 eem. liquid 
filtered off of the coagulated fibrinogen were heated during a quarter 
of an hour to 67—69° in a waterbath. The liquid remained perfectly 
clear till 64°; to obtain a coagulation as perfect as possible 5 eem. 
1°/, of a sulphas cupri solution were added as soon as the liquid 
became turbid; by this the coagulum became roughly floceulent and 
could easily be filtered off. The weight of the filtered fibringlobulin 
was afterwards determined in the same way as was done with the 
