( 616 ) 
coagulated fibrinogen and amounted to 0.1141 gram ashfree sub- 
stance; so from 300 eem. filtrate would have been gained 0.1369 
gram. In the liquid filtered off from the fibringlobulin no proteid 
could be demonstrated. 
Db). For comparison it was determined how much fibringlobulin 
the fibriogen solution used gave without treatment with Na FI. 
Therefore 100 eem. of this solution was again mixed with 200 ccm. 
4°/,°/, salt through which volume and salt quantity in this experiment 
was made equal with that of experiment «). By heating for a quarter 
of an hour to 55— 60° the fibrinogen was coagulated and was 
treated further as mentioned above; the weight of the fibrinogen 
amounted to 0,4548 gram ashfree substance. 250 cem. of the liquid 
filtered off from the coagulated fibrinogen were heated for a quarter 
of an hour to 67-—69 ; the liquid remained perfectly clear till 64°, 
just as in experiment a); here also 5 eem. 1°/, Cu 50, were added 
as soon as the first turbidness became visible. 
The coagulated fibringlobulin was filtered off and treated as men- 
tioned above; the weight of the fibringlobulin amounted to 0,1354 
gram; in the liquid filtered off from the coagulated fibringlobulin no 
proteid could be shown. 
Taking together the results of these experiments we find, that in 
experiment qa) after the removal of the precipitate obtained with 
Na FI, 0,2881 gram fibrinogen and 0,1369 gram fibringlobulin were 
present; and in experiment 5) 0,4548 gram fibrinogen and 0,1625 
gram fibringlobulin. So the quantity of fibrinogen was in experiment 
52 ; 
a) reduced to 100 through precipitation with Na Fl while the quantity 
- 
85 
of fibringlobulin only showed reduction to 100: Consequently there 
must have passed a considerable quantity of fibringlobulin into 
the filtrate after precipitation with Na Fl. The difference of 0,0256 
gram between the quantities of fibringlobulin, found in both experi- 
ments, must be attributed, apart from any errors of determination 
to the fact that the fibrinogen which was not precipitated with Na Fl 
is not perfectly free from fibringlobulin; the weight of this precipitate 
amounted to 0,2435 gram; if we abstract from this 0,0256 gram as 
being fibringlobulin, this precipitate contained to 100 mg. at 55° 
coagulable fibrinogen 11,7 mg. fibringlobulin; in experiment 6) 35,7 
mg. fibringlobulin was found to 100 mg. at 55° coagulable fibrinogen 
and in experiment a) after removal of the precipitate obtained with 
Na Fl 57,5 mg. fibringlobulin to 100 mg. at 55° coagulable fibrinogen. 
By precipitating with Na Fl the fibrinogensolution was consequently 
