( 617 ) 
divided into a precipitate, which contained relatively little and a 
filtrate which contained relatively much fibringlobulin. 
In experiment a) about half of the fibrinogen was precipitated 
with NaFl; the liquid poured off from this precipitate was clear; 
if however such a liquid is left standing for some time it becomes 
turbid and a new precipitate has formed itself after 24 hours, in 
the filtrate of this precipitate a new turbidness forms again ete., 
till at last after some days all the fibrinogen has precipitated. It 
may me expected after the above mentioned experiments, that, as 
more fibrinogen precipitates, relatively (that is to say with regard 
to the quantity of fibrinogen which was not precipitated) more 
fibringlobulin will be present in the filtrate; this supposition is 
confirmed by the two following experiments. 
1. 100 eem. horsefibrinogensolution of 0.642 °/, were precipitated 
with 200 cem. saturated natrium fluoride solution ; the precipitate was 
removed with a glass rod and the liquid remained standing after 
that twice 24 hours. When the fibrinogen, precipitated after that 
time, also was removed by filtering, the quantity of fibrinogen and 
fibringlobulin in 250 eem. filtrate was determined in the same way 
as in the above mentioned experiment a). It appeared that in these 
250 eem. 0.0742 gram fibrinogen and 0.1113 gram fibringlobulin 
were present. 
2. 100cem. of the same fibrinogensolution were precipitated with 
200 eem. saturated natrium fluoride solution; after removal of the pre- 
cipitate the liquid remained standing for eight days; putridity did 
not occur from this on account of the quantity of Na Fl, the new 
formed precipitate was filtered off, the filtrate became again turbid 
and after 24 hours a slight precipitate had again formed, that was 
filtered off. The filtrate was neutralised with a few drops of diluted 
acetic acid; by heating of the neutral liquid to 55—60° there followed 
only an exceedingly slight opalescence; the fibrinogen was therefore 
precipitated almost completely by the Na Fl; when the opaline liquid 
was filtered a considerable flocculent precipitate was formed by 
heating the filtrate to 67—69°. 
While in experiment a) after the removal of the precipitate obtained 
with NaFl still 1°/, times more fibrinogen than fibringlobulin was 
present in the filtrate, the analogous filtrate in experiment 1) con- 
tained only *, times as much fibrinogen as fibringlobulin, while in 
experiment 2) with a considerable quantity of fibringlobulin only 
a small quantity of fibrinogen was present. 
The results of the above described experiments lead to the con- 
clusion that at the coagulation of the fibrinogen, the fibringlobulin 
