( 619 ) 
Apart from this that then it would not be explained why the 
fibrinogen precipitated with NaFl does not produce any fibringlo- 
bulin by heating, it would have to be expected according to this 
view that, if the fibrinogen were precipitated with Nall for the 
second or third time also a part of it would be changed into fibrin- 
globulin, which ought to be found in the filtrate. This however is 
not the case; under these circumstances only very little or no fibrin- 
globulin is found in the filtrate. 
So, when it should be assumed, that the fibringlobulin is present 
in the fibrinogen solutions beforehand already, the question remains, 
whether this proteid is combined with the fibrinogen or must be 
considered as a simple admixture. 
For a compound plead some experiments of HAMMARSTEN ‘), in 
which is demonstrated that from concentrated fibrinogen solutions 
„after heating to 56—60° and filtering off of the coagulum, relatively 
less fibringlobulin is obtained than from the same solutions after 
their having been diluted. If the fibringlobulin were only an admixture 
it would be expected that the relation between the quantities of 
fibrinogen and fibringlobulin would always be the same; on the 
other hand, if the fibringlobulin is combined with the fibrinogen the 
results of HAMMARSTEN could be explained thus, that in diluted fibri- 
nogen solutions the fibringlobulin is more easily disintegrated. To a 
compound also points the fact, that when a fibrinogen solution is 
precipitated for the first time with NaFl a not inconsiderable quantity 
of fibringlobulin is precipitated also. 
Against a compound speaks however that by precipitating with 
NaF the fibringlobulin passes into the filtrate, at least for the greater 
part, for it is difficult to believe, that addition of alkali salt, as 
NaFl up to a quantity of about 3°/, would have for its result a 
splitting off of fibringlobulin. The following observation may perhaps 
give some light. 
100 eem. of horsefibrinogensolution were precipitated with the double 
volume of saturated natrium fluoride solution; the solution of NaFl 
used reacted almost neutral by exception ; with litmuspaper the alkaline 
reaction was hardly perceptible. Part of this natrium fluoride solution 
was now made weakly alkaline by addition of 0.8 eem. normal 
sodium hydrat with 200 eem. of the natrium fluoride solution ; with 
this 100 cem. of the same fibrinogen solution were precipitated twice 
in the same way. 
From the precipitates obtained with neutral and with alkaline 
NaF! two fibrinogen solutions of equal concentration were prepared. 
1) Loe. cit. 
