( 620 ) 
The fibrinogen was in both cases coagulated and filtered off by 
heating to 55—60°. The filtrate of the fibrinogen prepared with slight 
alkaline natrium fluoride solution gave a slight precipitate by heating 
to 70° or by addition of picric acid, while the precipitate of fibrin- 
globulin in the other filtrate was clearly greater, perhaps twice or 
three times. 
From this it would follow that the supposed splitting off of 
fibringlobulin is not brought about by Na Fl but by the alkaline 
reaction of the natrium fluoride solutions ; for this disintegration however 
exceedingly small quantities of alkali are already sufficient, for also 
that fibrinogen solution which was prepared with almost neutral 
Na Fl produced much less fibringlobulin than a fibrinogen solution 
of the same concentration, not prepared with Na FJ]. The supposition 
that water also, in particular at a rising temperature could bring 
about the splitting off of fibringlobulin is obvious; if this is the 
case there would be present in a fibrinogen solution a compound of 
fibringlobulin with fibrinogen, which is disintegrated more or less 
by hydrolysis and this idea is, as appears to me, most easily recon- 
ciled with the facts. The disintegration will in this case with raised 
temperature e.g. at 55—60’ be rather complete; from diluted solutions 
relatively more fibringlobulin may however be obtained than from 
concentrated solutions, because in the first case the disintegration 
will be more complete owing to the greater excess of water. That 
not all the fibringlobulin passes into the filtrate by the precipitation 
with Na Fl, becomes clear if only a partly hydrolytic disintegration 
is accepted. 
If the fibringlobulin is mixed simply with the fibrinogen in conse- 
quence of hydrolysis, be it then for a part only, it cannot be expected, 
— with a view to this, that by half saturation with salt as is usual 
with the preparation of fibrinogen, no complete precipitation of the 
fibringlobulin takes place, — that in every fibrinogensolution the 
relation between the quantities of fibrinogen and fibringlobulin will 
be the same; this may perhaps lead to the explanation of some 
observations of HAMMARSTEN *) from which it appeared that fibrinogen- 
solutions prepared from different plasma produce, it is true, relatively 
different quantities of fibringlobulin, that however a diluted solution 
does not always produce relatively more fibringlobulin than a con- 
centrated solution. 
In conclusion I will diseuss some facts here, relating to clotting 
by means of ferment. 
1) loc. cit. p. 456. 
