( 258 ) 



have had any influence of' the coagulative action of the rennet- 

 ferment, 



TABLE VI. 



Coagulation is visible: 



by A after 3 minutes; by A' after 3 minutes. 



>, B „ 2 „ „ B' „ 2 



„ C „ 1 „ „ C „ 1 



» D „ lVs „ „ D' „ H/s „ 



» E „ 2Vs » „ £' „ 21/2 „ 



This experiment shows that the distribution of chymosin (including 

 pro-chymosin) runs parallel with that of pepsin [including pro-pepsin), 

 a result agreeing with the researches of others. 1 ) 



Secondly the experiment shows the action of the rennet in corre- 

 sponding parts of the two symmetrical halves of (he stomach to 

 be equal. 



5. Distribution of enterokinase in the intestinal 

 mucous membrane. 



On the mucous membrane of the duodenum, jejunum, ileum, 

 coecum and colon, cut open length-wise,agar-eolumns were placed, 

 their contents being 3 cc. These having been left on it for 24 hours, 

 the agar-agar was cut fine and extracted with 3 cc. of a Ka Fl- 

 solution of 2 %. Then 6 cc. of diluted pancreatic juice were added 

 to '2 cc. of the filtrate. The former had been obtained by pressing 

 the pancreas eland of a newly killed pig, mixing the thick juice 

 thus obtained with a Na Fl-solution of 2 %. and filtrating the 

 mixture. 



In the mixture of 6 cc. diluted pancreatic juice thus obtained and 

 2 cc. agar-filtrate two albumen tubes were placed. 



By the side of this, controlling-experiments were made with 2 cc. 

 agar-filtrate and 6 cc. of Na Fl solution, instead of 6 cc. of the diluted 

 pancreatic juice. The digestion of the albumen was noted down 

 after 19 and 44 hours. 



!) Nencki u. Sieber. Zeitschr. f. physiol. Chemie 32 1901 S. 291 ; Pekelharing. 

 Ibkl. 35 1902 S.8 ; Pawlow u. Parastschuk. Ibid 42 1904 S. 415; Sawjalow. 

 Ibid 46 1905 S. 307. 



