( 260 ) 

 TABLE VIII. 



Liquid 



Digestion 

 after 



Experiment I 



Experiment II 



5ccagar+2cc 



intest. extr, ; 



ofthis2X3cc 



cut up; each 



3 cc mixed 



with 2 cc 



water; of this 



2X1 cc mixed 



each with2cc 



pancreatic 



juice 



I 

 4 hours 1+1+1 + 1=4 m.m. 1 + 1 f 1 + '■% = 37 4 m.m 



16 hours 



25 hours 



3Vs-r 41/3-r 3i/3+4=15V» m.m. 3V 2 +3U +3 Vs+4=14V> m.m. 



6 + 5 + 6 + 5 = 22 m.m. I 5 + 5 4 6 + 5 = 21 m.m. 



5 cc water + 



'ice intest. extr. 



of this 2X3 cc; 



each 3 cc 



mixed with 

 2 cc of water; 

 of this 2X1 cc 



mixed each 

 with 2cc of 



pancreatic 

 juice. 



4 hours 



16 hours 



25 hours 



l'.4+l'A+l + l=4Vnm.m 



4 4 4i/ 2 + 4 + 4=16i/s m.m. 



6 + 5 +5 + 6 = 22 m.m. 



l'4+l + l l /s + l'4 = 5 m.m. 



4 + 3V 3 + 4 + 41/2 = 17 m.m. 



5 + 5 + 5 + 6 = 21 m.m. 



This table leaves no doubt, but the method of extracting the agar 

 with water, gives reliable results. They turn out to be the same as 

 if the agar itself were water. The enterohinase must distribute itself 

 equally over agar and water. 



We observed the same with pepsin. 



Finally we shall describe a series of experiments, showing that 

 erepsin too enters the agar, and that this supplies us with a means 

 of determining its distribution over the intestinal mucous membrane. 



6. Distribution of Erepsin. 



The 2°/ agar used was not dissolved in water, but in NaFl of 

 2°/ because the amount of erepsin, passing from the intestine into 

 the agar during the time taken up by the former experiments, was 

 not great enough. 



Therefore it was expedient to leave the agar for at least 24 hours 

 on the mucous membrane, taking care to prevent putrefaction as 

 much as possible. 



