GAMETOGENBSIS OF SACCOCIRRUS 5 



fragments which pass through the nuclear membrane. The 

 droplets from the sperm tail shrink up and leave the periphery 

 of the oocyte quite clear, while the nucleolar fragments grow 

 and multiply and fill the egg with yolk. Buchner is not 

 altogether happy in his work on Saccocirrus oogenesis, and is 

 very cautious with regard to yolk-formation. He ends his 

 research with the statement, ' Die Dotterbildung von Sacco- 

 cirrus verdiente wohl eine eingehendere, mit Hilfe aller zu 

 Gebote stehenden Farbungen und Eeaktionen ausgefiihrte 

 Analyse '. 



No observer has hitherto given any account of Golgi body 

 or mitochondria in the oogenesis of Saccocirrus, and this form 

 has up to the present time resisted the efforts aimed at a satis- 

 factory interpretation of the problems which its oogenesis 

 presents. 



3. Technique and Material. 



Sections of a large collection of Saccocirrus were prepared 

 according to the plans given in my recent paper on technique 

 (13). The Mann-Kopsch and Champy-Kull methods were 

 particularly valuable. All the other techniques in current use 

 were tried. The material used was sent to me from Plymouth, 

 two lots in the month of June, another in July. 



The worms were cut into pieces after having been killed by 

 being dropped whole into a capsule full of fixative. Great 

 difficulty was experienced in making successful preparations 

 of the Golgi apparatus. All the first trials were unsuccessful, 

 but for some unknown reason all the latter attempts succeeded 

 completely both with Mann-Kopsch, Cajal, and Da Fano 

 methods (13). 



I have to thank Dr. Allen, F.E.S., of the Plymouth Biological 

 Laboratory, for sending me the material desired by me. 



Professor E. S. Goodrich, F.E.S., of. Oxford, kindly placed 

 at my disposal some of his own material of Saccocirrus 

 papillocercus from Naples, and sections cut from this 

 proved very useful. 



