GAMETOGENBSIS OF SACCOCIRRUS 



19 



a large lower zone. The upper cap is formed of delicate 

 granules which I think are fatty yolk and probably of 

 the Golgi elements ; these granules will go yellowish green 



Text-fig. 1. 



,CL. 



-n 



Centrifuged oocyte of Raccocirrus. Shows an upper layer of greenish 

 oily yolk (ul), clear middle layer with nucleus (n), lower layer 

 principally nucleolar deutoplasm (nl) with an upper layer mainly 

 mitochondrial (m). At cl are the cortical lamellae of the egg 

 membrane. (Chrome-osmium and Benda stain.) 



after prolonged osmication. The middle layer generally 

 shows two zones — an upper just beneath the fatty yolk and 

 staining in crystal violet, or iron haematoxylin, and looking 

 much like thickened cytoplasmic reticula ; then there is the 



C 2 



