GAMETOGENESIS OF SACCOCIRRUS 23 



glycogen, for this was generally easy, but in the identification 

 of chromatin; the problem was whether basophile chromatic 

 material was chromatinic ; this is the great problem of cytology 

 at the present time. 



I believe that I may be forgiven for holding an optimistic 

 view with reference to our present and future understanding 

 as to the behaviour of the Golgi elements and mitochondria 

 during oogenesis : I think that the works of Jan Hirschler, 

 Weigl, Nussbaum-Hilarowitz, Kio-Hortega, and my own 

 series of papers on the cytoplasmic inclusions have gone far 

 to shed a clear light on the subject, but I do at present feel 

 much puzzled over the nuclear phenomenon in oogenesis.^ 



One is driven onward trying to avoid the pool of Charybdis, 

 formed by the chromosome theorists who will not admit of 

 true chromatinic extra-nuclear extrusions, and the rock of 

 Scylla, which in my mind is constituted by the fact that it is 

 at times difficult to believe that the so-called extra-nuclear 

 extrusions are not chromatin. This special matter is further 

 discussed below under the heading of ' The Supposed Chroma- 

 tinic Nature of Extruded Nucleolar Material '. 



In all probability were it not for the ingenious, and one 

 must say believable theories of chromosome workers of Mor- 

 gan's, Wilson's, or McClung's schools, one would have no 

 hesitation in saying that the extra-nuclear extrusions were 

 chromatin, even though they frequently do not stain quite like 

 the chromatin of the ' resting ' nucleus. When one takes the case 

 of the secondary nuclei of the Hymenopterous egg it is very 

 difficult to avoid the conclusion that such granules are chromatin. 



This is a matter to which I have given a good deal of atten- 

 tion. Quite recently I have again gone over my Apanteles 

 material, and I have found an example which shows the 

 chromatin filaments at the diplotene stage of the prophases 

 of the heterotypic division, while the nucleolus is separate 

 and shows buds, some of which have already passed into the 

 cytoplasm to form minute secondary nuclei. 



1 Mr. R. J. Ludford's recent work has gone far to clear up parts of 

 this obscure ground ('Jour. Roy. Micr. Soc.', 1920-21). 



