154 



REGINALD JAMES LUDFORD 



the best results were obtained by adopting Cajal's method (4) 

 with shghtly longer fixation than he describes. Ovaries of 

 Dytiscus were fixed for about eight hours in Cajal's standard 

 fixative, and after rapid washing in distilled water were left 

 in silver nitrate solution for three days. Prepared slides were 

 stained by safranin or carmine. The Golgi body then appears 

 as a number of black granules in a pink-coloured cytoplasm, 

 the mitochondria when visible are usually brown in colour, 

 and the nucleoli of the cells are red. 



Text-figs. 1-4. 



t3| 

 -.,. ^#-f ^ 



1 



Follicle cells of the ovary of Dytiscus margin a lis. The 

 upper cell-wall is in each case in contact with the oocyte wall. 

 ge, elements of the Golgi body (Dictyosomes) ; in, mitochondria ; 

 n, nuclear membrane ; 7il, nucleolus (plasmosome). 



In the text-figures are shown the various stages of amitosis. 

 At fig. 1 is seen the so-called ' resting stage ' of the cell. It 

 will be observed that there is a single nucleolus within the 

 nucleus, and scattered through the cytoplasm are the darkly- 

 impregnated elements of the Golgi body, while the mito- 

 chondria are more or less evenly distributed in the cell. In the 

 stage shown in fig. 2 the nucleus has elongated and the nucleolus 

 is dividing into two. The Golgi elements still remain scattered 

 in the cytoplasm, but it will be noticed they show a tendency 

 to lie near the nuclear membrane — a tendency which is apparent 

 in the other figures. At a later stage, as shown in fig. 3, the 



