518 E, N. PAVLOVSKY AND E. J. ZAKIN 



following tV-niients : diiistasc!, invertaso, and a proteolytic 

 ferment dissolving fibrin and splitting peptone. These are 

 essentially all the data to be had in literature on the question 

 discussed. 



Methods used in Preparing the Material for 

 Chemical Investigation of the Ferments. 



The only lit material is presented by live bees, live for 

 anatomical purposes. They are chloroformed and dissected 

 in physiological solution (0-75 per cent.) of common salt for 

 the preparation of the intestine. The removed intestine is 

 washed in a Petri dish with the physiological solution and 

 separated into the following parts : crop, stomach, small and 

 large intestines. 



Each portion is further dissected in order to remove its 

 contents, washed in a fresh portion of the same solution, 

 and placed in a small evaporating glass with a small quantity 

 of desiccated sand and several drops of glycerine. After the 

 portions of the intestine of all the bees have been placed in 

 glasses, they are ground with a glass mortar until a uniformly 

 opaque emulsion is produced. Then to each glass is added the 

 necessary quantity of glycerine or some other liquid, the whole 

 is rapidly mixed and poured out into a jar with a hermetically 

 closing glass stopper. 



Extracts were prepared with (a) glycerine, (b) distilled water, 



(c) a mixture of equal quantities of the liquids named, and 



(d) physiological solution of common salt. As an antiseptic 

 a few (5-10) drops of toluol were added. The jars were 

 several times shaken thoroughly and left to stand in the dark- 

 ness at the temperature of the room for different periods. 



The density of the extracts varied. We started from strong 

 extracts, ninety bees per 30 c.c. of liquid, i.e. from each portion 

 of the intestine taken from ninety bees an extract was prepared 

 in 30 c.c. By degrees as the experiments progressed it proved 

 to be more practicable to take weaker extracts, until finally we 

 stopped at the proportion of 25 bees per 50 c.c. of liquid. 



