520 



E. N. I'AVLOVSKY AND E. J. ZAKIN 



Catalase. 



As is known, catalast' is a ferment widely distributed in the 

 animal and vegetable kingdom. Regarding its presence in the 

 body of the bee no data are known in the literature. 



For the determination of catalase we employed a special 

 apparatus constructed by one of us (Zarin). 



The process consisted in mixing 2 c.c. of corresponding 

 extracts of ferments with H c.c. of water ; to the filtered 

 mixture were added 10 c.c. -of freshly prepared 1 per cent, 

 solution of hydrogen peroxide ; the number of c.c. of oxygen 

 evolved being marked after the expiration of twenty-four hours. 

 It need not be mentioned that all the analyses were accom- 

 panied by control experiments. The results obtained in the 

 investigations are shown in Table II. 



rp 



Table II. Catalase in the Intestine of the Bee. 



A'w. 



1 

 2 

 3 



4 

 5 



a 



7 



8 



9 



10 



II 



12 

 13 



U 



15 



Dale of 

 Experi- 

 ment. 



8 IV 1917 

 23 VI 1917 

 16x1 1917 



1 IV 1918 



15iv"l918 



22 IV 1918 

 24 IV 1918 

 19 Yi 1918 

 2\Til918 



4 vn 1918 



7 vm 1918 

 21 vni 1918 



Concentratio7i and 

 CinnjKisition of 

 Extract. 

 60 bees : 25 c.c. glyc. 

 30 bees : 12-5 c.c. glyc 

 30 bees : 15 c.c. glyc.-j- 

 15 c.c. water 



'> >> 



30 bees : 30 c.c. water 

 15 bees : 7-5 c.c. glyc.+ 

 7*5 c.c. water 



25 bees : 25 c.c. glyc.+ 

 25 c.c. water 



>> >> 



15 drones : 7-5 c.c. glyc. 



+ 7-5 c.c. water 

 25 bees : 25 c.c. glyc.+ 



25 c.c. water 



Larije 

 hUe^tiiui. 



2-0 











3-5 

 20 

 3-3 



2-8 



0-3' 















u 





 

 



' In experiment no. 8 the bees were taken three hours after the hives were removed 

 from the hibernating quarters, when the contents of the rectum were discharged by them. 



It is seen from the table that catalase is a specific secretion 

 of the stomach in the bee and drone. This portion of the 



