542 E. N. PAVLOVSKY AND E. J. ZARIN 



(2) Alkaline solution of casein. To 10 c.c. of 1 per cent, 

 solution of casein containing 1 in 200 c.c. 10 drops of 10 per cent, 

 solution of soda were added 2 c.c. of extracts of ferments 

 tested ; the test-tubes with the mixture were placed for one 

 hour in water at a temperature of 88-40° C, after which 

 a 0-5 per cent, solution of citric acid was added to their contents 

 by drops evoking a turbidity in the solution of undigested 

 casein. 



By applying the methods described we obtained the same 

 results in all cases, trypsin being established only in the 

 stomach of the bee and drone, whilst the remaining parts of 

 the intestine were devoid of it. 



Chymosin. 



Regarding the presence of chymosin in the organism of the 

 bee there are no data in literature. 



For its determination we used a mixture of 10 c.c. of milk + 

 90 c.c. of water+ 1 c.c. of 10 per cent, calcium chloride. 



To 10 c.c. of this mixture was added 1 c.c. of extracts of the 

 ferment tested, and the liquid was placed in a water-bath 

 at 40° C. 



The extracts from the stomach always caused milk to 

 coagulate ; the formation of the coagulum in the experiments 

 conducted being observed not earlier than after two, and 

 not later than after fifteen minutes. In the test-tubes contain- 

 ing extracts from the remaining portions of the intestine the 

 milk did not coagulate during three hours, after which the 

 experiment was stopped. 



Thus our experiments show that the stomach of the bee 

 and drone contains abomasum ferment of considerable activity. 



E m u 1 s i n . 



For the determination of the ferments splitting the glucosides 

 we used 1 per cent, solutions of amygdalin, salicin. and arbutin. 

 The results obtained were negative. It was impossible at that 

 time to purchase andromedotoxin, which produces the toxic 

 properties of honey as well as any other glucosides. 



